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. 2016 Nov 14;5(11):e269. doi: 10.1038/oncsis.2016.67

Figure 1.

Figure 1

Notch3 expression in various breast cancer cell lines and the correlation between Notch3 and EMT. (a) Notch3 expression levels in various human breast cell lines, including MCF-7, T47D, SKBR3, MDA-MB-231 and BT549, were tested via western blotting. (b) Confirmation that Notch3 positively regulates E-cadherin expression via western blotting by overexpressing or knocking down Notch3 in MDA-MB-231 cells. (c) Confirmation that ectopically overexpressed Notch3 inhibits EMT by qRT-PCR. (d) Confirmation that knock down of Notch3 promotes EMT by qRT-PCR. Data are presented as the mean±s.d. of three experiments. *P<0.05, **P<0.01 and ***P<0.001 (Student's t-test) as compared with control cells.