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. 2016 Nov 14;5(11):e269. doi: 10.1038/oncsis.2016.67

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ChIP and luciferase assays confirm that Kibra is regulated by Notch3 at the transcriptional level. (a) The ChIP assay used normal IgG (IgG) or anti-Notch3 antibody to determine whether Notch3 can bind the RBP-Jk binding site in the Kibra promoter in MDA-MB-231 cells. After ChIP, PCR shows that the Notch3/RBP-Jk does not bind to the RBP-Jk binding site in the sense orientation. (b) After ChIP, PCR reveals that the Notch3/RBP-Jk complex binds to the RBP-Jk binding site in the antisense orientation. (c) MDA-MB-231 cells were co-transfected with pCLE, pCLE/N3, pGL-Wt-Luc or pGL-Mut-Luc. All cells were also co-transfected with a Renilla luciferase plasmid. Luciferase activity was normalized to that of Renilla. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 (Student's t-test) as compared with control cells. Data are presented as mean±s.e.m. (n=3).