Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 14;5(11):e269. doi: 10.1038/oncsis.2016.67

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Functional relationship between Notch3 and Kibra in breast cancer epithelial cells. (a) After shKibra constructs are transfected into MCF-7 cells, the expression level of Notch3 is significantly reduced. Correspondingly, significantly elevated vimentin and decreased E-cadherin expression levels are seen as compared with those of MCF-7 cells transfected with shNC. However, the expression of Notch1 is unchanged. (b) RT-PCR shows that the expression levels of Notch3 and E-cadherin mRNA are downregulated in MDA-MB-231 cells after shKibra#6 and #8 are transfected, whereas the expression level of vimentin was upregulated. (c) Nevertheless, when Kibra overexpression constructs are transfected into MDA-MB-231 cells, the expression of Notch3 is slightly upregulated (or unchanged), and the expression levels of vimentin and E-cadherin have no significant changes. The expression of Notch1 is only slightly inhibited. (d) RT-PCR analysis reveals that the expression levels of Notch3 and E-cadherin mRNA are upregulated in MDA-MB-231 cells, whereas the expression of vimentin mRNA is reduced when Kibra overexpression constructs are transfected into MDA-MB-231 cells. Data are presented as the mean±s.d. of three experiments. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 (Student's t-test) as compared with control cells.