| Notch3 |
5′-CTGCTGTTGGACCACTTTGC-3′ |
5′-CTTTGAGGCCAGGGAGGAAG-3′ |
| Notch1 |
5′-CGCTGACGGAGTACAAGTG-3′ |
5′-GTAGGAGCCGACCTCGTTG-3′ |
| Kibra |
5′-AGCTCCAAGTATGACCCTGAG-3′ |
5′-AAAGCCACGCTCTTTGAACTG-3′ |
| Merlin |
5′-TTGCGAGATGAAGTGGAAAGG-3′ |
5′-CAAGAAGTGAAAGGTGACTGGTT-3′ |
| Ex |
5′-CCACCTCTTTGGACTCAGTGT-3′ |
5′-CAAATTGGTCGATACCCTTGCT-3′ |
| CTGF |
5′- ACCGACTGGAAGACACGTTTG-3′ |
5′- CCAGGTCAGCTTCGCAAGG-3′ |
| Tead |
5′-GGCCGGGAATGATTCAAACAG-3′ |
5′- CAATGGAGCGACCTTGCCA-3′ |
| Cyr61 |
5′-CTCGCCTTAGTCGTCACCC-3′ |
5′-CGCCGAAGTTGCATTCCAG-3′ |
| Wwtr1 |
5′-GATCCTGCCGGAGTCTTTCTT-3′ |
5′- CACGTCGTAGGACTGCTGG-3′ |
| E-cadherin |
5′-AAAGGCCCATTTCCTAAAAACCT-3′ |
5′-TGCGTTCTCTATCCAGAGGCT-3′ |
| RBP-Jk |
5′-CGGCCTCCACCTAAACGAC-3′ |
5′-TCCATCCACTGCCCATAAGAT-3′ |
| Hes-1 |
5′-TCAACACGACACCGGATAAAC-3′ |
5′-GCCGCGAGCTATCTTTCTTCA-3′ |
| Delta |
5′-GATTCTCCTGATGACCTCGCA-3′ |
5′-TCCGTAGTAGTGTTCGTCACA-3′ |
| Hey-1 |
5′-ATCTGCTAAGCTAGAAAAAGCCG-3′ |
5′-GTGCGCGTCAAAGTAACCT-3′ |
| Crumb3a |
5′-CAGGTGCCTCTCAAATTCTTGC-3′ |
5′-ACAGGAACCAATGGTAGTTTCAC-3′ |
| Vimentin |
5′-GACAATGCGTCTCTGGCACGTCT-3′ |
5′-TCCGCCTCCTGCAGGTTCTT-3′ |
| GAPDH |
5′-TGGACTCCACGACGTACTCAG-3′ |
5′-ACATGTTCCAATATGATTCCA-3′ |
| Luciferase assay: wild-type CSL-binding site |
5′-CGAGCTCGACCCAACTGGGCTTCATT-3′ |
5′-TCCCCCGGGGGACCCATCTCCTCTTACATCTGTG-3′ |
| Luciferase assay: mutated CSL-binding site |
5′-GGCAAAAAAGGGTTAAATCTCAGTACACAGATGTAA-3′ |
5′-TTACATCTGTGTACTGAGATTTAACCCTTTTTTGCC-3′ |
| ChIP primers flanking RBP-Jk-binding site in forward orientation |
5′-GACCCAAACAGAGGAAGTGTAG -3′ |
5′-GGCCCTCCTTCATTCCATAAA-3′ |
| ChIP primers flanking RBP-Jk-binding site in reverse orientation |
5′-ACCCAACTGGGCTTCATT-3′ |
5′-CCCATCTCCTCTTACATCTGTG-3′ |
| shNotch3
|
5′-CACCGTATAGGTGTTGACGCCATCCACGCATT
CAAGAGATGCGTGGATGGCGTCAACACCTATATTTTTTG-3′ |
5'-GATCCAAAAAATATAGGTGTTGACGCCATCCAC
GCATCTCTTGAATGCGTGGATGGCGTCAACACCTATAC-3′ |
