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. 2004 Jun;135(2):947–958. doi: 10.1104/pp.103.036376

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Copyright © 2004, American Society of Plant Biologists

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Figure 3. — Lipase subcellular localization. A, Western blot of total protein isolated from organs of 6-week-old wild-type Arabidopsis plants. Lane 1, leaf; lane 2, stem; lane 3, flower; lane 4, silique; lane 5, seed. B, Western blot of cytosolic and microsomal membrane fractions isolated from the rosette leaves of 3.5-week-old wild-type Arabidopsis plants. Lane 1, cytosol; lane 2, microsomal membranes. C, Western blot of cytosol, chloroplasts, and chloroplastic subfractions isolated from the rosette leaves of 3.5-week-old wild-type Arabidopsis plants. Lane 1, broken chloroplasts; lane 2, intact chloroplasts; lane 3, chloroplastic membranes (thylakoids and envelope); lane 4, stroma; lane 5, cytosol. The blots were probed with lipase-specific antibody.