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Lipase expression during leaf development and senescence. A, Northern blot of total RNA isolated from the rosette leaves of wild-type Arabidopsis plants at various stages of development. The blot was probed with the 3′ untranslated region of the lipase cDNA. Lanes 1 to 5: 2-, 3-, 4-, 5-, and 6-week-old tissue, respectively. Each lane contained 10 μg of RNA. B, Corresponding ethidium bromide-stained agarose gel of the fractionated RNA.
