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. 2016 Nov 7;5(11):e268. doi: 10.1038/oncsis.2016.69

Figure 4.

Figure 4

SALL4 regulates CD44 expression in gastric cancer cells. (a) Schematic image of luciferase reporter vectors under the control of CD44 promoter. (b) HEK293T cells were co-transfected with CD44 luciferase reporter and SALL4 plasmid. The luciferase activity was evaluated by using dual-luciferase reporter system. (c) SALL4 knockdown gastric cancer cells were transfected with CD44 luciferase reporter as indicated. The luciferase activity was evaluated by using dual-luciferase reporter system. (d) MGC80-3 cells were co-transfected with CD44 luciferase reporter and SALL4 siRNA. The luciferase activity was evaluated by using dual-luciferase reporter system. (e) MGC80-3 cells were co-transfected with CD44 luciferase reporter and different concentrations of SALL4 siRNA. The luciferase activity was evaluated by using dual-luciferase reporter system. (f) ChIP-PCR analyses of SALL4 binding to CD44 promoter in MGC80-3 cells. (g) ChIP-PCR analyses of SALL4 binding to CD44 promoter in control and SALL4 knockdown MGC80-3 cells. *P<0.05, **P<0.01.