Table 2.
Tumor ID | Outcome | Days frozen | |
---|---|---|---|
No cooling | 24 h cooling | ||
HROC107 | M1(+/+); M2(+/−) | M3(−/+); M4(−/+) | 293 |
HROC118 | M1(−/+); M2(−/−) | M3(−/+); M4(−/+) | 174 |
HROC119 | M1(−/−); M2(−/−) | M3(−/−); M4(−/−) | 154 |
HROC122 | M1(−/−); M2(−/−) | M3(−/−); M4(−/−) | 130 |
HROC123 | M1(+/−); M2(−/−) | M3(−/−); M4(−/−) | 130 |
HROC125 | M1(−/−); M2(−/−) | M3(−/−); M4(−/−) | 74 |
HROC129 | M1(+/+); M2(−/+) | M3(−/−); M4(−/−) | 61 |
HROC130 | M1(−/+); M2(−/−) | M3(−/−); M4(−/−) | 57 |
HROC131 | M1(+/+); M2(+/+) | M3(+/+); M4(+/+) | 54 |
HROC135 | M1(−/+); M2(−/−) | M3(−/−); M4(−/+) | 40 |
Ø Matrigel | 6/20 | 2/20 | |
With Matrigel | 8/20 | 7/20 |
A total of 4 mice were implanted on both flanks with human CRC tissue samples. M denotes individual animals xenografted; the outcome is given in parentheses as index + (outgrowing tumor) or index − (no outgrowth). Tumor tissues presoaked with Matrigel are indicated in underlined signs. The overall number of successful sites with and without the addition of Matrigel is given for the total of 20 sides implanted.