Table 2. miRNAs and genes included in the network for which differential expression in PMF patients compared to healthy controls has been confirmed.
| PMF vs CTR |
CD34+ |
GR and Plasma | ||
|---|---|---|---|---|
| Arrays | RNA-seq | RT-PCR | RT-PCR (PMF vs CTR; RQ mean±dev) | |
| miR-106a-5p | UP | UP GR (1.9±1.2 vs 1.08±1.0) | ||
| miR-145-5p | UP | UP GR (1.7±0.9 vs 1.0±0.5) | ||
| miR-146b-5p | UP | UP | ||
| miR-155-5p | UP | UP | ||
| miR-17-5p | UP | UP GR (1.5±0.7 vs 1.1±0.9) | ||
| miR-185-5p | UP | UP GR (3.0±3.1 vs 1.5±1.2) | ||
| miR-195-5p | UP | UP | UP GR (15.3±10.5 vs 1.8±2.1) UP Plasma (12.4±17.5 vs 1.37±0.95) | |
| miR-19a-3p | UP | UP | UP GR (15.3±10.5 vs 1.8±2.1) UP Plasma (12.4±17.5 vs 1.37±0.95) | |
| miR-19b-3p | UP | UP | UP | |
| miR-21-5p | UP | UP | ||
| miR-29a-3p | UP | UP | UP | UP Plasma (3.0±3.1 vs 1.5±1.2) |
| miR-29c-3p | UP | UP | ||
| miR-494-3p | UP | UP | UP | |
| ANGPT1 | UP | UP | ||
| ARHGEF7 | DOWN | DOWN | ||
| CDC42 | DOWN | DOWN | ||
| MEF2D | DOWN | DOWN | ||
| SMAD7 | DOWN | DOWN | ||
Abbreviations: miRNA, microRNA; PMF, primary myelofibrosis.
The second column indicates if miRNAs and genes resulted up- or down-regulated according to array data used for network reconstruction. The following columns indicate for which elements a significant differential expression has been previously confirmed using RNA-seq in CD34+ cells,22 and using RT-PCR in CD34+ cells,20 in granulocytes (GR) and in plasma samples.