Figure 6.
Unmodified hAAT exerts anti-inflammatory effect on BMDM–RMA cocultures. BMDMs (1 × 106 cells per well in triplicate) were pretreated with hAAT (0.5 mg/ml); after 24 h BMDMs were cocultured with RMA cells (2.5 × 105 cells per well) with or without stimulation with IFNγ and IL-1β (both at 10 ng/ml). RMA cells were diluted 1:4 after 48 h, and after 72 h (A) cells were washed and stained for flow cytometric analysis, and (B) supernatants were examined for IL-6 and NO levels. Mean ± SEM, *p < 0.05. Unpaired two-tailed Student’s t-test was employed to assess differences between groups.