Figure 8. Impact of Net4 on disruption of the laminin network and its effect on vascularization.

(a) Diagram indicates vessel diameter distribution (left) within Net4-FL^E195A,R199A-treated CAMs (blue squares), and the Net4-FL group (red triangle). Model showing the common established capillary hierarchy formed within the CAM. Numbers indicate sizes of different vessels in the common established hierarchy (middle). The table displays the diameter of different hierarchical vessels from Net4-FL^E195A,R199A- (blue) and Net4-FL-treated (red) experiments (right) (n.p., not present). (b) Ultrastructural analyses of Net4-FL^E195A,R199A- (blue) and Net4-FL-treated (red) CAMs using transmission electron microscopy. Images show the capillary endothelium surrounded by perivascular cells. The endothelium E is shown and PVCs are indicated as P. Electron microscopy images highlight the vascular basement membrane (vBM, red arrow heads (middle) and red asterisks (bottom)) between the endothelium and PVCs, which are surrounded by a vBM layer. (c) Model of Net4 effect on capillary cell types (endothelium (Endoth.) and pericyte (Pericyte)) and the vBM. Scale bars (b), 2 μm (top); 1 μm (middle); 0.5 μm (bottom).