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. 2004 Jun;135(2):1027–1039. doi: 10.1104/pp.103.037739

Figure 2.

Figure 2.

Other members of the Pcr gene family confer Cd(II) resistance in yeast. A, Dendrogram of the AtPcr family in Arabidopsis. The tree was generated based on amino acid sequence homologies using CLUSTALW (origin2). B, Growth in the presence or absence of Cd(II) of ycf1 yeast cells expressing the empty pYES2/NTC vector (V), AtPcr1, AtPcr2, AtPcr8, AtPcr9, AtPcr10, or OsPcr1. The cells were grown on 1/2 SG medium with or without 30 μm Cd(II). C, Hydropathy profile of AtPcr1. Hydrophobicity was determined by the method of Kyte and Doolittle, using a moving window of 11 amino acid residues. D, The amino acid sequences of the Pcr protein in Arabidopsis (AtPcrs 1, 2, 8, 9, and 10) and rice (OsPcr1). Identical amino acid residues are shown by black boxes. CLUSTALW program was used for this alignment. An asterisk marks regions that are highly conserved among the Pcr family members.