Figure 6.

AtPcr1 enhances Cd(II) resistance in Arabidopsis plants. AtPcr1-sense (A) and AtPcr1-antisense (B) lines on the Col-0 background were germinated and grown on 40 or 50 μm Cd(II)-containing 1/2 MS agar plates for 4 weeks. Wild-type (wt) Col-0 was grown as a control (A). The AtPcr1 activation-tagged line and its wild-type control on the WS background (C) were also grown in identical conditions. To determine the AtPcr1 message levels, total RNA was extracted from whole plants grown and RT-PCR was performed using the Pcr1-R1 and V5 tag-primers (A), AtPcr1- or AtPcr2-specific primers (B and C) and tubulin primers for the internal standard (A–C).