Figure 8.

AtPcr1-expressing yeast cells reduce their Cd content by decreasing Cd(II) uptake. A, Cd content in Cd-treated yeast cells expressing the empty vector or AtPcr1. The cells were grown in 15 μm Cd(II)-containing SG media for indicated times, harvested, briefly rinsed twice with ice-cold water, and digested with 65% HNO₃ at 200°C for 6 h. Their Cd contents were measured using stomic absorption spectroscopy. Shown are averages ± se (n = 3). B, Uptake of ¹⁰⁹Cd(II) by yeast cells expressing empty vector (EV) or AtPcr1 that had been preincubated for 6 h with 20 μm CdCl₂. See “Materials and Methods” for a description of the experimental details.