Figure 9.

Antisense Arabidopsis protoplasts show elevated Cd contents. A, Cd uptake by protoplasts from wild-type, sense, and antisense Arabidopsis plants was assessed by following the protocol described in “Materials and Methods.” The protoplasts were isolated, purified, and concentrated by a percoll gradient. Shown are averages ± se (n = 6). B, AtPcr1 and AtPcr2 transcript levels in the Arabidopsis plants used in A. To amplify both the AtPcr1 and AtPcr2 messages, the Pcr1-R1 and Pcr1-X1 primers were used for the PCR step of RT-PCR.