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. 2004 Jun;135(2):1113–1128. doi: 10.1104/pp.103.036749

Figure 1.

Figure 1.

Responsiveness of Arabidopsis cell cultures and seedlings to flg22 elicitor. A, Extracellular medium alkalinization in Arabidopsis cell culture. The pH of the cell culture extracellular medium was measured with glass electrode. White boxes represent control cell cultures and black boxes represent flg22-treated cell cultures. Error bars correspond to sd observed in two independent experiments that were used for the microarray analysis. B, RT-PCR of AtWRKY29 (At4g23550) and AtMPK3 (At3g45640) in Arabidopsis cell culture. RT-PCR of a constitutively expressed actin gene (At5g09810) was also performed to control equal cDNA amount in each reaction (bottom lane). C, Ethylene production in Arabidopsis seedlings. Increase of ethylene was measured by gas chromatography. White boxes represent control seedlings and black boxes represent flg22-treated seedlings. Error bars correspond to sd. D, RT-PCR of AtWRKY29 (At4g23550) and AtMPK3 (At3g45640) in Arabidopsis seedlings. RT-PCR of a constitutively expressed actin gene (At5g09810) was performed to control equal cDNA amount in each reaction (bottom lane).