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. 2004 Jun;135(2):1113–1128. doi: 10.1104/pp.103.036749

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Copyright © 2004, American Society of Plant Biologists

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Figure 4. — Temporal expression patterns of Arabidopsis ACRE orthologs. Semiquantitative RT-PCR transcript profiling of AtACRE genes of Ler suspension cells (A) and Col-0 seedlings (B) challenged with ±flg22 peptide for 0, 30, and 60 min and for 0, 10, 30, and 60 min, respectively. AtACRE genes (from top to bottom): AtACRE1a (At5g47230), AtACRE1b (At4g17490), AtACRE4 (At5g17680), AtACRE31 (At4g20780), AtACRE74a (At5g37490), AtACRE74b (At1g66160), AtACRE111 (At4g25470), AtACRE126 (At5g15130), AtACRE132 (At3g16720), AtACRE189 (At5g67250), AtACRE231 (At3g28340), AtACRE264a (At2g05940), AtACRE264b (At5g35580), AtACRE276 (At1g29340), AtACRE284a (At2g30020), AtACRE284b (At4g08260), AtACRE284c (At2g40180), and AtCPK1 (At5g04870). RT-PCR of a constitutively expressed actin gene (At5g09810) was performed to control equal cDNA amount in each reaction (bottom lane).