Figure 4. Time course of relative viral genome accumulation in the leaves of infected rice plants.

Total RNA was extracted from leaf sheaths collected at the indicated times. Samples were analysed for viral genome accumulation by quantitative reverse transcription polymerase chain reaction. Primers specific for genome segment 10 of SRBSDV and genome segment 8 for RRSV were used to quantify the relative viral genome titre. The gene encoding the rice U6 small nuclear RNA was used as an internal standard. Each data point represents the mean ± standard deviation of three biological replicates.