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. 2016 Dec 7;6:37927. doi: 10.1038/srep37927

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Effect of Ucf-101 on HAX-1 expression in H9C2 cells overexpressing mitochondrial Omi/HtrA2. Effect of either Ucf-101 (b) of CsA (c) upon the XIAP expression in H9C2 cells overexpressing mitochondrial Omi/HtrA2. Both the H9C2 cell line stably with eGFP (Vector) and H9C2 cell line stably with mitochondrial Omi/HtrA2 (Full-Omi) were treated with either 0.1uM Ucf-101(Omi/HtrA2 inhibitor) or 0.1 μM cyclosporine A (CsA, mitochondrial permeability transition pore inhibitor) for 24 hours. Representative Western blots showing cytosolic XIAP or HAX-1 expression. GAPDH was used as a loading control. n = 6 each. *p < 0.05 versus Vector; ^#p < 0.05 versus Full-Omi.