Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 7;6:38536. doi: 10.1038/srep38536

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Proteins derived from whole cell lysate, membrane fraction, and cytosolic fraction of the cells maintained in media with differential calcium levels, including low-calcium (with 0.2 mM calcium), normal-calcium (with 1.8 mM calcium), and high-calcium (with 20 mM calcium) media (details are provided in Supplementary Table S1) for 3-h were subjected to Western blotting of annexin A1. GAPDH served as the loading control for whole cell lysate and cytosolic fraction, whereas E-cadherin served as the loading control for membrane fraction. Full-length blots of these cropped images are presented in Supplementary Figure S2. (B) Band intensity was quantitated using ImageQuant TL software (GE healthcare). Each bar represents mean ± SEM from 3 independent experiments. ^†p < 0.05 vs. normal-calcium and high-calcium conditions; ^‡p < 0.05 vs. normal-calcium and low-calcium conditions.