Figure 1.
Changes in signal transduction upon microRNA-124 (miR-124) overexpression in human epidermal growth factor receptor 2 (HER-2)-positive cells. (A) SKBR3, T47D, and MCF7 breast cancer cells were analyzed by Western blotting for HER2 and estrogen-receptor expression. Tubulin was used as a loading control. (B) MiR-124 expression of in SKBR3, T47D, and MCF7 cells by realtime polymerase chain reaction. (C) MiR-124 expression in SKBR3 cells following transfection with miR-124 duplex or scramble control. (D) Significantly altered signaling pathways upon miR-124 overexpression. Histogram shows the log2 (fold changes) of the reporter activities of different signaling pathways. The data are presented as the mean ± standard deviation (SD) of three independent experiments. *P < 0.001.