Figure 2.

Signal transducer and activator of transcription 3 (Stat3) was a direct target of microRNA-124 (miR-124). (A) The potential duplexes formed between miR-124 and Stat3 3′-untranslated region region. The sequence of the mutant vector lacking the miRNA biding site is also shown. (B) SKBR3 were transiently transfected with the indicated constructs. Dual luciferase activity was measured 48 h post transfection. (C) Western blotting analysis of Stat3 expression in SKBR3 following transfection with miR-124 duplex or scramble control. The data are presented as the mean ± standard deviation (SD) of three independent experiments. *P < 0.001.