Figure 3.

MicroRNA-124 (miR-124) overexpression enhances sensitivity to irradiation. (A) Western blotting for cleaved-poly (ADP-ribose) polymerase (PARP), signal transducer and activator of transcription 3 (Stat3), and p-Stat3 in SKBR3 cells transfected with miR-124 or control and treated with irradiation or not treated with irradiation. (B) Flow cytometry analysis of cell death in cells transfected with scramble or miR-124 and treated with irradiation. Cells were stained with Annexin V and propidium iodide. (C) Percentage of cell death (B). Data are shown as the mean ± standard deviation (SD) of three independent experiments **P < 0.001).