Figure 3. Comparison of the kinetics of interaction of PEG 1500 (left) and PEG 3400 (right) with the α-hemolysin nanopore as a function of temperature at +50 mV in 3 M KCl buffer.

(Top) Blockade duration versus temperature. Dashed lines are exponential fits. (Down) Blockade frequency versus temperature. Dashed lines are exponential fits. For each temperature, the data point corresponds to the mean blockade duration (top) or to the mean blockade frequency (down) estimated from a single exponential fit of the blockade duration distribution and of the inter-blockade duration distribution respectively. Error bars correspond to the uncertainty of the fitting values (see the Methods section and Supplementary Fig. S1).