Table 1. GC-MS quantification of free PG, PG released from enzymatic digestion of phlorin and total PG (mg/g) using PG standard.
| Lines | PG (mg/g lyophilized tissue) |
||
|---|---|---|---|
| Free PG | Conjugated PG (phlorin) | Total PG | |
| 19–2 (chloroplastic) | 5.7 (15.2%) | 31.76 (84.8%) | 37.46 |
| 23–2 (cytosolic) | 0.28 (23.5%) | 0.91 (76.5%) | 1.19 |
The unconjugated PG was quantified as described in the methods section. In addition, an equal amount of the extract was also digested with rapidase enzyme and the total PG was quantified. The amount of conjugated PG was calculated by subtracting the free form from the total.