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. 2016 Dec 7;6:38630. doi: 10.1038/srep38630

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Copyright © 2016, The Author(s)

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(a) Antimicrobial assay of supernatants and cell lysates of E. coli BL21 (DE3) after induced by IPTG for 5, 10, 20 h. E. coli cells are transformed with (1) pET28a-bovA; (2) pET28a-bovAM; (3) pET28a-bovAM + pACYC-Duet-bovT₁₅₀; (4) pET28a-bovAM + pACYC-Duet-bovT; (5) pET28a-bovA + pACYC-Duet-bovT₁₅₀M. M. flavus NCIB8166 was used as the indicator strain for antimicrobial assay. (b) MS analysis of cell lysates of E. coli BL21 (DE3) co-transformed with pET28a-bovA and pACYC-Duet-bovT₁₅₀M. (c) MS analysis and antimicrobial assay of supernatants of E. coli C43 (DE3) co-transformed with pET28a-bovAM and pACYC-Duet-bovT after induced by IPTG. A, culture supernatants; B, cell lysates.