Table 1. Advantages and disadvantages of next-generation sequencing ^{31, 32, 33, 34, 35} .

Advantages	Disadvantages
High throughput Able to multiplex Lower cost Automated analysis Uses less DNA Can run in parallel with other genetic tests	Higher start-up cost Mutation-positive test may require Sanger sequencing for confirmation Complex workflow Dedicated data storage and analysis required Reduced sensitivity for large insertions/deletions >20 base pairs