Table 2. Examples of next-generation sequencing platforms as of May 2014.
| Platform | Read length (bp) and mode | Run timea (hours) | Number of reads (single or paired; millions) | Data yield (Gb per run) |
|---|---|---|---|---|
| Illumina MiSeq v2 chemistryb | 2 × 150 paired | ~24 | 24–30 (paired) | 4.5–5.1 |
| Illumina MiSeq v3 chemistryb | 2 × 75 paired | ~21 | 44–50 (paired) | 3.3–3.8 |
| Illumina MiSeq v3 chemistryb | 2 × 300 paired | ~56 | 44–50 (paired) | 13.2–15.0 |
| Ion Torrent PGM, 316 Chip v2c | Up to 200 single | 3 | 2–3 (single) | 0.3–0.5 |
| Ion Torrent PGM, 318 Chip v2c | Up to 200 single | 4.4 | 4.0–5.5 (single) | 0.6–1.0 |
| Ion Torrent PGM, 318 Chip v2c | Up to 400 single | 7.3 | 4.0–5.5 (single) | 1.2–2.0 |
| Ion Torrent Proton with PI Chipc | Up to 200 single | 2–4 | 60–80 (single) | Up to 10 |
| Illumina NextSeq 500 (mid output)b | 2 × 150 paired | 26 | Up to 260 (paired) | 32.5–39.0 |
| Illumina HiSeq 2500 (rapid run single flow cell)b | 2 × 150 paired | 40d | Up to 300 (paired) | 75–90 |
a
Upstream preparatory work not taken into consideration.
d
Run time based on dual flow cell.