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. 2004 Jul;16(7):1741–1752. doi: 10.1105/tpc.021980

Figure 5.

Figure 5.

Replication/Accumulation of PSTVdNB- and PSTVdNT-Derived Mutants in Systemic Leaves of Biolistically Inoculated Tobacco Plants.

(A) Surface and transverse sectional views of tobacco leaves show transient expression of dimeric GFP, upon biolistic bombardment of plasmids containing CaMV 35S:dGFP, in the epidermis (Ep). The bombarded plants are transgenic for the expression of dimeric dsRed in the phloem (Ph). The images show that biolistic bombardment does not deliver cDNAs into cells below the epidermis. Bars = 40 μm.

(B) RNA gel blot shows accumulation of PSTVdNB, PSTVdNT, and their mutants in systemic leaves of bombarded plants. c-(+)PSTVd and l-(+)PSTVd denote circular and linear (+)-PSTVd, respectively. Bands of 5S rRNA show equal loading of RNA in all lanes. Lane 0, mock inoculation; lane 1, PSTVdNT; lanes 2 to 4, PSTVdNT-derived mutants G201U, U315C, and A309U; lane 5, PSTVdNB; lanes 6 to 8, PSTVdNB-derived mutants U201G, C315U, and U309A; lane 9, PSTVdNTA47U/U313A; lane 10, PSTVdNBU47A/A313U.