Table 1.
Line | Weak | Medium | Severe | Variable in One Cell | Wild Type | Total |
---|---|---|---|---|---|---|
2 | 0 | 2 | 0 | 1 | 4 | 7 |
G6 | 1 | 1 | 2 | 1 | 11 | 16 |
M7 | 0 | 2 | 0 | 5 | 4 | 11 |
M11 | 3 | 3 | 0 | 3 | 3 | 12 |
M12 | 1 | 1 | 0 | 0 | 5 | 7 |
Total | 5 | 9 | 2 | 10 | 27 | 53 |
Isolated mesophyll cells were prepared using three different fully expanded leaves of 30-d-old plants. For each sample, at least 200 cells were observed. Because each plant could display a variety of phenotypes depending on the observed cell, the category of each plant was assigned using the most severe phenotype represented. Weak, 40 to 60 chloroplasts per mesophyll cell; medium, 10 to 40; severe, 1 to 10; variable in one cell, numerous normal chloroplasts together with one to four larger ones (5 to 20 times larger in section compared to the wild type).