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. 2004 Aug 15;18(16):1952–1957. doi: 10.1101/gad.1198404

Figure 4.

Figure 4.

Depletion of APC1 by RNA interference induces G2/M arrest and apoptosis in the absence of p53. (A) Immunoblot analysis of H1299 cells transfected with 0, 0.05, and 0.5 μg siRNAs directed against either Lamin A/C or APC1. Tubulin was monitored as a loading control. (B) Crystal violet staining of H1299 cells following two rounds of transfection with Lamin A/C or APC1 siRNAs, or mock-transfected. (C) Cell viability assays of H1299 cells 48 h following transfection of siRNAs, or mock-transfected. (D) Annexin V-FITC staining to monitor apoptosis in siRNA-transfected cells. (E) Cell-cycle analysis (left) and quantitation of the percentage of cells arrested in G2/M (right) of siRNA-transfected cells.