Figure 4.
Effects of Gαs on mitochondrial biogenesis and oxygen consumption in lipid-rich 3T3-L1 cells. (A) Oxygen consumption of lipid-rich 3T3-L1 cells infected with adenoviral vectors expressing the various factors indicated. Each bar represents the mean ± S.E.M. (n = 4). (*) p < 0.05 between the two groups of mice indicated. (B) Electron micrograph of lipid-rich 3T3-L1 cells infected with adenoviral vectors expressing Gαs protein. (Right) Higher magnifications of the rectangle-enclosed areas shown at left. (L) lipid droplets; (N) nucleus. Black arrowheads show representative mitochondria. (c) Light micrograph of lipid-rich 3T3-L1 cells infected with adenoviral vectors expressing Gαs proteins. Each micrograph shows both light (left) and dark (right, fluorescent) fields in parallel. Black and white arrowheads point to the same cell shown in light and dark fields, respectively. The numbers on the left of the micrograph indicate the number of days after adipogenic induction.