Fig. 3.

Global heterozygosity for Ctgf impairs pregnancy-induced β-cell proliferation but does not impact β-cell hypertrophy or β-cell mass. A and B: quantification and colabeling of pancreas sections with insulin (green) and Ki-67 (red) to quantify proliferating β-cells in virgin and gestational day 14.5 (GD14.5) wild-type and Ctgf^LacZ/+ females. DAPI is in blue. Arrows indicate proliferating β-cells. Scale bar, 100 μm. C: individual β-cell size in virgin and GD14.5 wild-type and Ctgf^LacZ/+ females. D: β-cell mass measured in virgin, GD14.5, and gestational day 16.5 (GD16.5) samples. Error bars represent standard deviation. Samples with the same letter are not significantly different from each other (P < 0.05).