Figure 2.

The effects of bumped kinase inhibitor 1517 (BKI-1517) in SCID/beige mice infected with Cryptosporidium parvum. The number of parasites shed per 25 mg of stool in experimental groups was quantified using real-time polymerase chain reaction analysis, normalized to a standard curve. Samples were quantified at different days after infection, with the initial time points indicating oocyst levels before treatment. The median number of oocysts shed by each experimental group are indicated with solid black bars. Curves illustrate infection levels. A, Mice were treated with 60 mg/kg BKI-1517 once daily (solid triangles; n = 6), with 20 mg/kg morning and 40 mg/kg evening daily (solid circles; n = 7), or with vehicle control (hollow squares; n = 7). B, Mice were treated with BKI-1517 at doses of 60 mg/kg once daily (solid circles; n = 6), 120 mg/kg once daily (solid triangles; n = 6), or vehicle control (hollow squares; n = 6).