LC-MS/MS analysis of HETEs in sEH+/+ and sEH−/− heart perfusate at baseline (preischemia) and directly after 15-s ischemia (post-ischemia). All detected HETEs (5-, 11-, 12-, and 15-HETE), were decreased postischemia (after perfusion was reinstated) compared with baseline in both sEH+/+ and sEH−/− mice (P = 0.02, P = 0.04, P = 0.05, and P = 0.03 respectively; A–D). Only 12-HETE in sEH+/+ mice did not reach statistically significant level (P = 0.09). There was no difference in any of the measured HETEs between sEH+/+ and sEH−/− mice (P > 0.05). *P ≤ 0.05 vs. baseline sEH+/+. #P ≤ 0.05 vs. baseline sEH−/−. n = 7 sEH+/+, n = 10 sEH−/−.