Fig. 1.

γHV-68 early replication is increased in the lungs of CCR2^−/− mice. A: at the indicated time after infection, lungs were harvested and infectious virus was quantified by plaque assay on NIH 3T12 monolayers (mean ± SE), n = 4–5 mice per group, repeated once with same result. Two mice were below the detection threshold in the B6 10 dpi group and are not represented on the graph. B: isolated alveolar epithelial cells were infected at multiplicity of infection of 1.0. At the indicated time, cell culture supernatants were harvested and viral replication determined by plaque assay (n = 3 per group, repeated 3 times with similar results). Statistical significance was calculated by Student's t-test, *P < 0.05. C and D: H&E staining of FFPE sections from infected B6 or CCR2^−/− mice at 7 dpi. E and F: γHV-68-infected B6 or CCR2^−/− lung sections at 21 dpi. Images representative of 3–4 mice per group.