Fig. 11.

Recruitment of granulocytes is altered in CCR2^−/− BMT mice following γHV-68 challenge. A–C: flow cytometric analysis of single-cell suspension prepared from collagenase-digested lung tissue at 7 dpi. Autofluorescent CD11c+ alveolar macrophage were first gated out (not shown) after which cells were gated by CD45, CD11B double positivity (A, top right quadrant). Subsequent gating was carried out using staining for LY6C and LY6G (B) and for CX3CR1 and MHC II (C). D: MHCII+ cells from C were back gated onto the LY6G/LY6C plot from B and are highlighted in red (shown as a percentage of CD45+,CD11B+ cells). Shown are concatenated plots combining data from all mice in a particular group, n = 4–5 mice per group (repeated once with similar results).