Fig. 12.

Treatment of BMT mice with αGR1 antibody reduces but does not eliminate granulocyte infiltration in response to γMHV-68. A: flow cytometry plots showing staining of single-cell suspensions prepared from collagenase-digested lung tissue at 7 dpi. GR1, CD11B double positive cells were first gated (top row) after which subsequent gating was carried out using LY6C and LY6G (bottom row). B through D: quantification of inset gates a, b, and c from LY6C/LY6G staining in A (n = 4–5 mice per group, repeated once with similar results); statistical analysis was carried out by ANOVA; *P < 0.05, **P < 0.01, ****P < 0.0001. E: representative images of differential stained lung leukocytes from collagenase-digested lungs.