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. 2016 Jul 22;311(3):L611–L627. doi: 10.1152/ajplung.00193.2016

Fig. 2.

Fig. 2.

CCR2−/− mice recruit neutrophils to the lungs instead of monocytes in response to γHV-68 infection. A: representative dot plot showing side scatter (granularity) and forward scatter (cell size) flow cytometry profiles of collagenase-digested lung cells from B6 or CCR2−/− mice infected for 7 days with γHV-68. B: quantification of total leukocyte numbers present in the lungs at 7 dpi. C: quantification of differential stains showing numbers of leukocyte subpopulations present in the lungs at 7 dpi. D: representative images of differential stained collagenase-digested lung cells at 7 dpi. E: flow cytometric analysis of collagenase-digested lung cells at either 7 or 21 dpi stained for the indicated cell surface marker. Graphs display means ± SE, n = 4–5 mice per group (repeated twice with similar results), **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with B6.