Fig. 5.
Recruitment of neutrophils is increased in response to γHV-68 following CCR2−/− BMT. Mice at 5 wk post-BMT or control nontransplanted mice were infected for 7 days with 5 × 103 pfu γHV-68. A–C: numbers of lung infiltrating leukocyte populations as assessed by differential stain of single-cell suspensions from lung homogenates. D and E: qRT-PCR gene expression analysis of the neutrophil chemoattractant genes CXCL1 and CXCL2. F: lung-associated viral titer as assessed by plaque assay on 3T12 fibroblast monolayers. Statistical significance was determined by ANOVA (A–E) or Student's t-test (F), *P < 0.05, **P < 0.01, ***P < 0.001, n = 4–5 mice per group (repeated twice with similar results).