Fig. 1.

Activation of EP3 in the thick ascending limb (TAL) attenuates cyclooxygenase 2 (COX-2) protein expression induced by hypertonic saline. COX-2 protein expression was determined by Western blot analysis in medullary (m) TAL tubules isolated from vehicle and sulprostone (sulp)-treated rats. Rats were given 1% NaCl in the drinking water for 3 days after pretreatment for 3 days with either vehicle or sulp. COX-2 increased in response to hypertonic NaCl compared with control and was decreased in TAL tubules from rats treated with sulp. The data in the histogram are presented as means ± SE; n = 3.