Fig. 5.
Glomerular injury and apoptosis in the kidneys of saline- or Ang II-infused mice. A, top: glomerular capillary density shown by Von Willebrand factor (vWF) immunostaining; middle: podocytes shown by WT1 staining; bottom: apoptosis shown by cleaved caspase-3 (CC3) immunostaining. B: representative images of glomerular capillary tuft quantification by converting color image to binary image using ImageJ software, as described in the materials and methods. C: quantification of glomerular capillary density through vWF staining in A. *P < 0.05 compared with all of the other groups. D: quantification of WT1-positive cells. *P < 0.05 compared with all of the other groups. E: quantification of CC3-positive cells. *P < 0.05 compared with normal saline-infused groups; ^P < 0.05 compared with Ang II-infused HBendo−/− mouse kidneys. HPF, high-power field.