Fig. 7.
Endothelial HB-EGF deletion reduced Ang II-induced renal inflammation. A: F4/80 staining indicated macrophage infiltration in the HBlox/lox control kidneys after Ang II infusion, whereas only sparse F4/80-positive cells were detected in the HBendo−/− kidneys. B: compared with Ang II-infused kidneys from HBendo−/− mice, Ang II-infused HBlox/lox kidneys showed increased interstitial staining of iNOS, an M1 marker, and weak positivity for arginase I, an M2 marker. C: infiltration of CD3-positive lymphocytes was seen in the periglomerular and perivascular regions in Ang II-infused HBlox/lox control kidneys but was rarely detected in Ang II-infused HBendo−/− kidneys. D: some of the infiltrating CD3-positive lymphocytes also expressed p-EGFR. E: more CD4- vs. CD8-positive cells were detected in the perivascular infiltration areas in both Ang II-infused groups, with HBlox/lox control kidneys having significantly higher numbers of both CD4- and CD8-positive infiltrated cells.