Table 1.
Summary statistics of C. irritans transcriptome
| Tomont | Theront | Trophont | |
|---|---|---|---|
| Sequencing | |||
| Raw reads (pair-end) | 79,350,336 | 66,432,722 | 123,624,738 |
| Clean reads (pair-end) | 73,151,792 | 62,228,442 | 109,567,740 |
| Clean ratio | 92.19% | 93.67% | 88.63% |
| Assembly | |||
| Primary UniGene | Final UniGene | ||
| No. of unigenes | 80,078 | 49,104 | |
| Total length (bp) | 76,079,839 | 50,174,026 | |
| N50 length (bp) | 1,302 | 1,338 | |
| Mean length (bp) | 1,002 | 1,022 | |
| Largest transcripts (bp) | 17,074 | 17,074 | |
| Eliminating contamination | |||
| No. of clean unigenes | |||
| Not T. ovatus | 31,893 | ||
| Not prokaryote CDS (≥ 100 aa)a | 9,253 | ||
| Annotation | |||
| NCBI-Nr | 8,569 | ||
| No. of best hits among 6 ciliate reference databases | Total no. of the reference databases | ||
| I. multifiliis | 1,551 | 8,097 | |
| P. peptides | 2,036 | 39,519 | |
| T. borealis | 1,867 | 21,943 | |
| T. elliotti | 1,332 | 22,562 | |
| T. malaccensis | 1,252 | 26,378 | |
| T. thermophila | 1,215 | 26,996 | |
aCDS (≥ 100 aa): only used the CDS region more than 300 bp