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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Leuk Lymphoma. 2016 May 17;57(10):2409–2416. doi: 10.3109/10428194.2016.1161186

Figure 2A and B. Tris-DBA induced apoptosis in CLL B-cells involves poly (ADP ribose) polymerase (PARP) cleavage and is caspase dependent.

Figure 2A and B

A. Purified CLL B-cells (4.0 × 106/ml) were treated with Tris-DBA at sub-lethal dose of 4μM. Cell lysates were then analyzed for PARP cleavage in Western blot using a specific antibody which recognizes both the native (116 kDa) and cleaved (86 kDa) forms of PARP. B. CLL B-cells were treated with Tris-DBA at a dose of 4μM with or without pre-treatment of the cells with the pan-caspase inhibitor Z-VAD-fmk. Cell lysates were then analyzed for the PARP-cleavage via western blot. Actin was used as a loading control. CLL patients are indicated by arbitrary numbers (P1 and P2).