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CLL B-cells (1.0 × 106/ml) were cultured with human primary bone marrow stroma for 24 h as described by us before, where Tris-DBA was then added at increasing concentrations (0, 1, 2.5, 7.5 and 10 μM), and then CLL B-cells were harvested and analyzed for apoptosis by staining with annexin/PI.
