Fig. 3.

Direct downstream targets of Akt signaling in response to unloading and irradiation. A: representative immunoblots of GSK3β^S9, total GSK3β, and GAPDH in gastrocnemius muscle (top); quantification of phosphorylated to total GSK3β protein expression (bottom). B: representative immunoblots of PRAS40^T246, total PRAS40, and GAPDH in gastrocnemius muscle (top); quantification of phosphorylated to total PRAS40 protein expression (bottom). C: representative immunoblots of TSC2^T1462, total TSC2, and GAPDH in gastrocnemius muscle (top); quantification of phosphorylated to total TSC2 protein expression (bottom). D: representative immunoblots of FOXO3^S253, total FOXO3, and GAPDH in gastrocnemius muscle (top); quantification of phosphorylated to total FOXO3 protein expression (bottom). E: representative immunoblots of FOXO1^S256, total FOXO1, and GAPDH in gastrocnemius muscle (top); quantification of phosphorylated to total FOXO1 protein expression (bottom). All samples were run on the same gel. Values are means ± SE. Statistical significance was set at P < 0.05. Data are normalized to LB controls. Dashed lines indicate adjacent lanes were cropped for representative immunoblots. #Main effect of HS; &main effect of irradiation; †significantly different from all groups.