Fig. 4.
Mammalian target of rapamycin complex 1 (mTORC1) signaling in response to unloading and irradiation. A: representative immunoblots of P70S6KT389, total P70S6K, and GAPDH in gastrocnemius muscle (top); quantification of phosphorylated to total P70S6K protein expression (bottom). B: representative immunoblots of rpS6S240/244, total rpS6, and GAPDH in gastrocnemius muscle (top); quantification of phosphorylated to total rpS6 protein expression (bottom). C: representative immunoblots rpS6S235/236, total rpS6, and GAPDH in gastrocnemius muscle (top); quantification of phosphorylated to total rpS6 protein expression (bottom). All samples were run on the same gel. Values are means ± SE. Statistical significance was set at P < 0.05. Data are normalized to LB controls. Dashed lines indicate adjacent lanes were cropped for representative immunoblots. #Main effect of HS; &main effect of irradiation.