Fig. 2.

Inflammatory mediators are increased in the midbrain of Tollip-deficient mice 6 h after LPS intra-nigral injection. a Eleven-month-old female mice underwent PBS (1 μL) or LPS (0.1 μg in 1 μL) injection (n = 7 and n = 8 respectively for WT mice and n = 6 and n = 12 respectively for the Tollip KO mice). mRNA amounts in midbrain extracts are normalized by β-actin and GAPDH. b IL-1β protein quantification by ELISA on 60 μg of midbrain protein extracts from the injected (LPS 0.1 μg) and non-injected sides. n = 5 for WT and 7 for KO 11-month-old mice. Box-and-whisker graphs show median, min., and max. values and 25th and 75th percentiles. Plots represent individual values and means are represented as a “+.” *P < 0.05; **P < 0.01; ***P < 0.001 versus PBS for a. and versus non-injected side for b. Two-way ANOVA followed by a Tukey’s post hoc test