Fig. 5.

Nitrotyrosine immunostaining is increased in the midbrain of Tollip-deficient mice 6 h after LPS intra-nigral injection. a. Representative nitrotyrosine immunostaining in non-injected and LPS (0.1 μg) - injected side in WT and Tollip KO 15-month-old male mice. Magnification = ×40. Scale bar = 30 μm. b Quantification of fluorescence intensity performed on two fields at the injected area, on three sections per animal (n = 4 in WT and 5 mice in KO group). Box and whiskers show median, min., and max. values, 25th and 75th percentiles. Plots represent individual values and means are represented as a “+.” ^# P < 0.05; ^### P < 0.001 versus non-injected side and ^*** P < 0.001 versus WT mice. Two-way ANOVA followed by a Tukey’s post hoc test